Streptomyces sp.Y9343이 생산하는 glucanase의 대량배양조건에 관한 연구
The study of mass production of glucanase from Streptomyces sp. Y9343 for hydrolysis of dental plaque
윤정원, 신미정,
소속 상세정보
윤정원 ( Yoon Jeong-Weon ) - 수원대학교 자연과학대학 유전공학과
신미정 ( ) - 수원대학교 자연과학대학 유전공학과
KMID : 0355420000240030299
Abstract
For the development of novel dental supplies could by mass production of dental plaque degradable enzyme.
The purpose of study was to investigate the optimal condition of mass production of glucanase from Steptomyces sp. Y934, its produced high activity of dental plaque degradable glucanase. Our study was incresed of glucanase secretion from Streptomyces sp. Y9343 by different carbon source and its addition time after 3 days growth of the culture.
The obtained results were is as follows
1. For the purpose of mass production of glucanase from Streptomyces sp. Y9343 used insoluble glucan(from Streptococcus mutans), dextrin(from corn), dextran(from Leuconostoc mesenteroides) into culture medium, 1% dextrin produced high glucanase among. them. Further culture medium used dextrin because it´s economic carbon source.
2. An electron micrograph, the shape of spores are smooth and round surface, and rectiflexible chain of spores.
3. Glucanase production was increased in the stationary phase of 0.5% starch medium in which added 1% dextrin: after 3 days.
This result implies that the production of glucanase influence adding time of dextrin as inducer.
키워드
덱스트린;글루칸분해효소;불용성글루칸
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